Journal of chromatographic science,2015年53(10):1680-1684 ISSN：0021-9665
[Wan, Dingrong] Pharmaceutical School, South-Central University for Nationalities, Wuhan 430074, China email@example.com;[Que, Hongling; Liu, Diangang] Foreign Languages School, Hubei University of Traditional Chinese Medicine, Wuhan 430065, China;[Li, Luyang; Mei, Qing] Pharmaceutical School, South-Central University for Nationalities, Wuhan 430074, China;[Wan, Xiangluan] Luojia College of Wuhan University, Wuhan 430065, China
[Wan, Dingrong] South Cent Univ Nationalities, Pharmaceut Sch, Wuhan 430074, Peoples R China.
Hibisci mutabilis Folium (HMF), the dried leaf of Hibiscus mutabilis (Malvaceae), is commonly used in traditional Chinese medicine. This article aimed to establish a high-performance liquid chromatography method for the determination of rutin and isoquercitrin contents in the HMF, and to compare the content variation in all the samples, including nearly withered yellow leaf, in different collection periods, so as to provide research basis for the quality evaluation and determination of the optimal collection period. A reversed-phase HPLC separation method was employed, with a BDS Hypersil C18 column (4.6 m x 250 mm, 5 mum), under the following conditions: acetonitrile-0.3% phosphoric acid (15:85, v/v) solution as the mobile phase, flow rate 1.0 mL/min at 30 degrees C and detection wavelength 254 nm. The calibration curves for rutin and isoquercitrin were linear over the range of 1.5-48 and 0.25-8 mug/mL, and the average recoveries were 99.92 and 100.45% (RSD: 2.39% and 2.11%, respectively)]. Based on the analysis results, it was found that contents of rutin and isoquercitrin in HMF (mature green leaf) harvested in different periods had significant difference, and reached the highest in mid-December. It was also found that the contents of the two components in the mature green leaf were much higher than those in the nearly withered leaf from the same collection period. In conclusion, the results indicated that the HPLC method was easy-to-operate and precise, and could be applied for the determination of rutin and isoquercitrin contents in the HMF. The experimental data also showed that early winter should be the most suitable collection period for HMF.
Airway remodeling can lead to irreversible airflow obstruction and persistent airway hyper-responsiveness, which is the pathological basis of refractory asthma. To investigate the preventive effect of protocatechuic aldehyde on airway remodeling in asthmatic mice by lung morphometry methods. BALB/c mice were used to establish model of airway remodeling by ovalbumin (OVA) inhalation. Bronchoalveolar lavage fluid (BALF) were collected for eosinophils (EOS) count and detection of interleukin 4 (IL-4), interleukin-13 (IL-13) and interferon (IFN-gamma) content. The left lung pathological sections were performed HE, AB-PAS and Masson staining. The epithelial lamina thickness of the left main bronchus (Re), the smooth muscle layer thickness (Rm), the number of goblet cells and goblet cell area percentage (%Ac) and gas side of the road and vascular collagen deposition (%A(infinity), %A(vc)) situation were measured. Protocatechuic aldehyde gavage made the reduction of BALF EOS count. IL-4 and IL-13 levels also decreased, while the IFN-gamma level increased. The left main bronchus Re, Rm, goblet cell count, Ac% and A(infinity)% and A(vc)% reduced. Protocatechuic aldehyde can significantly control airway inflammation and prevent airway remodeling.
The body-part term, HAND, ranks 48 in Swadesh\'s 100-Word List.This paper discusses the origin and meanings, and then the rules of semantic development in the HAND semantic field by comparing with other languages.The word itself does not only denote the body part but also things resembling hands in shape, position, function and things associated with hands.Plenty of linguistic evidence can be found to illustrate that all human beings regard their bodies as the basis and starting point of recognition of the whole world.